Authors:
Chukwudebe, E.P., Bankole, S.O*, Asonibare, A.O
and Adedokun, S.A ,Nigeria |
Abstract:
Antimicrobial and phytochemical properties of the crude extract of some selected plant samples
against some microbial isolates was investigated.Plant samples were extracted using ethyl acetate
and methanol as extracting solvents based on polarity. Antimicrobial studies was carried out
using Agar disc diffusion method. Zones of inhibitions were measured, Tetracycline, Ampicillin,
Chloramphenicol and Ofloxacin antibiotics were used as positive control. Extracting solvent
served as negative control and work was done in triplicates.Plant samples were found to contain
several bioactive compounds in various combinations. Ethyl acetate Pawpaw leaves extract gave
the best results with zones of inhibition as high as 27.3 ± 11.7mm and 25.3 ± 0.6mm against
Escherichia coli and Candida albicans respectively, while methanol Pawpaw leaves extracts gave
the best result with zone of inhibition as high as 25.0 ± 13.0mm against Staphylococcus aureus.
Ampicillin and Chloramphenicol in all cases and for both extracting solvents showed no
significant difference in the mean zone of inhibition compared with plant extracts at 5% level of
significance. The minimum bactericidal concentration was found with ethyl acetate pawpaw
leaves extract against Escherichia coli and Pseudomonas aeruginosa (bactericidal) at 100mg/ml
and 200mg/ml respectively while all the others were bacteriostatic |
