Abstract:
Jasminum polyanthum is an important ornamental plant belonging to the Oleaceae family. Both
shoot tip and leaves were used as explant for the induction of shoots via indirect organogenesis.
For callus induction, explants were excised from field grown mature plants and thereafter
incubated on MS basal medium supplied by 1.0 mg/l BA supplemented with various
concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) at 0.0, 1.0, 2.0, 4.0 and 6.0 mg/l.
Maximum percentage of callus induction from both explants was procured by the application of
2,4-D at 2.0 mg/l. Significant release of callus induction was swelled from leaf explants when
compared to shoot tips at different 2,4-D levels. For the proliferation stage, induced callus was
transferred into MS supplemented with BA at 0.0, 1.0, 2.0 and 3.0 mg/l along with NAA at 0.0,
0.5 and 1.0 mg/l. The maximum percentage of shoot proliferation per culture was achieved from
callus cultured in 1.0 mg/L BA along with 0.5 mg/l NAA. For rooting, microshoots were treated
with either IBA or IAA at 0.0, 0.5, 1.0 and 2.0 mg/l. Maximum rooting formation (Number and
length) was recorded by the application of IBA at 2.0 mg/l. For acclimatization, the complete
plantlets grown in pots filled with peat moss + vermiculite was suitable for 91% plantlets to
survive. |
