Abstract:
Cymbopogon citratus and Cymbopogon nardus are two species of lemongrass very useful
because they have analgesic, anti-inflammatory, and hypotensive properties, used in the
development of popular medicines. Three experiments were carried out: organogenesis,
embryogenesis and in vitro conservation, achieving with these tools the establishment in vitro. In
the organogenesis three types of tissues were used: nodal segments, meristematic apices and
meristems. In embryogenesis, three 2,4-dichloro phenoxyacetic acid (2,4-D) (0, 2.0, 2.5, 3.0 mg /
L) proteins were used in the culture medium. For in vitro preservation, four mannitol proteins (0,
10, 20, 30 mg / L) were used in the culture medium. The results establish the in vitro
establishment of C. citratus and C. nardus through biotechnological techniques. Outbreaks
survived in all treatments of organogenesis, embryogenesis and in vitro conservation of
lemongrass, produced roots and proteins 85% survival during the acclimatization stage. The
acclimatized plants obtained a vigorous appearance and developed normally when they were
transferred to the soil. The vitroplants were preserved in vitro under conditions of slow growth
affected by mannitol, which retarded growth without affecting survival |
