Authors:
Jinette Ndonkou Nfozon*, Monique Odette Kamtchueng, Roland Nkwelle, Jennifer Orwa, Gabriel Oluga Aboge, Moukeu Raymond Simplice, Fabrice Fekam Boyom, Feudjio Ndemanou Yolande and Christopher B. Tume ,Cameroon
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Abstract:
This study aimed to evaluate the in vitro immunomodulatory activity of methanolic extracts of
Triplotaxis stellulifera and Crassocephalum vitellinum on different parameters on mouse
peritoneal macrophages. The effects of the extracts on peritoneal macrophage viability were
evaluated using the 3 (4, 5-dimethylthiazole-2-yl)-2, 5 diphenyltetrazolium bromide (MTT)
assay. They were further evaluated for the stimulation of nitric oxide (NO) production,
lysosomal enzyme activities and pinocytic activity of macrophages, and on macrophage
phagocytosis by the nitroblue tetrazolium dye reduction (NBT) assay.
The results showed that methanolic extracts of Triplotaxis stellulifera and Crassocephalum
vitellinum stimulated nitric oxide production by lipopolysaccharide-activated macrophages,
while no cytotoxic effects were observed. Both extracts significantly (P<0.05) increased, the
activity of lysosomal enzymes, NBT dye reduction and the pinocytic index of macrophages.
Furthermore, the methanolic extracts of these plants increased the phagocytic activity of both
Lipopoysaccharide-activated and non-activated macrophages.
These findings show that the extracts can be potent sources of immunomodulatory agents,
although further complementary studies are needed to confirm these finding |
