Abstract:
This project aimed to characterize the twenty most commercially cultivated rice varieties in the
Dominican Republic. These varieties are as follows: PROSEQUISA 4; PROSEQUISA 5;
PROSEQUISA 10; PROSEQUISA 15; Jaraguá; ISA 40; Juma-57; Juma - 67; Idiaf I; Idiaf II;
Idiaf III; Emeralda; Cristal 100; Yokahu; Puita; Ambar; Palmar 18; Pava Shienn; Quisqueya;
and Tempique, which, according to data obtained from Rice Development, Ministry of
Agriculture of the Dominican Republic, have a considerable planting area in the country. The
methodology that we used for characterizing or "fingerprinting" of these varieties is Single
Nucleotide Polymorphism or SNP. These markers are the most abundant and are distributed
throughout the genome. For the determination of SNPs, we used the primers developed by
different authors we used the protocols they suggested to run the PCR reaction and run 5 µl the
product on 2% agarose and compared for each variety. We found differences among some
varieties that could be used to part difference in any legal dispute.
|
