Authors:
Ajab Khan Tareen, Muhammad Anwar Panezai, Ashif Sajjad, Jahangir Khan Achakzai, Abdul Manan Kakar
and Nazima Yousaf Khan ,Pakistan
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Abstract:
We assessed total phenolic content (TPC), total flavonoid content (TFC), free radical quenching
capacities in terms of 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH), TAC (Total Antioxidant
Capacity), Hydrogen Peroxide Scavenging (HPS), ferric reducing Antioxidant power (FRAP),
acute and chronic toxicity assays and mineral contents in the peel and pulp of five varieties of
apple (Malus domestica Borkh.) fruit from Balochistan province. The peel extracts of tested
varieties exhibited significantly (p < 0.05) substantial amount of TPC, TFC and extraction yield,
predominantly in the peel of Red Delicious. In terms of half maximal inhibitory concentration
(IC50) values Katja pulp exhibited lowest values in all the in vitro antioxidant assays.
Luminescence-based assays showed that all the tested peel and pulp extracts exhibited toxicity
towards V. logei only at high concentration and maximum contact time. Furthermore, significant
correlation between TPC and DPPH, TAC, FRAP and HPS assays were found, demonstrating
that phenolics contribute to antioxidant capacity. Pertaining to the mineral analysis, both fruit
tissues revealed the concentration of K to be maximum, preceded by Ca, Na, Fe, Mn, Co, Cu and
Cd. Obtained results impart information on nutritional, antimicrobial and phytochemical
composition of analyzed apple varieties and thereby inspire their consumption and cultivation. |
